Daily Line Purge
If a solvent with salt or buffer content, or a solvent with a pH <5 or >13 is used on your system, it is recommended to purge the line at the end of each day using water and air. The line can then be re-primed during the next day with its designated solvent. The procedure is as follows.
Line Purging - End of Day Procedure
Place the solvent line that needs to be purged in an empty bottle/container.
Run the following method to purge the line with air, flush the line with water, and then purge the line with air again. This method can be saved on the system for future use.
Leave the solvent line in the empty container until the beginning of the next shift.
Action | Inlet 1 | Flow | Volume | Notes |
Prime w1 | Solvent # | 10 | 5 | Draw 5 mL of air through the solvent line and push through to top waste |
Wait | Manual Resume | Program will automatically pause. Transfer the solvent line from an empty container to a bottle of clean water. | ||
Prime w1 | Solvent # | 10 | 10 | Draw 10 mL of water through the solvent line and push through to top waste |
Wait | Manual Resume | Program will automatically pause. Transfer the solvent line back to the empty container. | ||
Prime w1 | Solvent # | 10 | 5 | Draw 5 mL of air through the solvent line and push through to top waste |
Re-Priming - Beginning of the Next Day Procedure
Place the solvent line back into the designated solvent and run the following method. This method can be saved on the system for future use.
The solvent line is now re-priming and ready for the next extraction.
Action | Inlet 1 | Flow | Volume | Notes |
Prime w1 | Solvent # | 10 | 3 | Prime the solvent line and valve 1 with 3 mL of designated solvent |
Prime w1 | Water | 10 | 5 | Clean valve 1 and the top waste line with 5 mL of water |
Air-purge w1 | Air | 10 | 5 | Purge valve 1 and the top waste line with 5 mL of air |