Daily Line Purge
All buffers should be purged at the end of each day using water and air. The line can then be re-primed during the next day with its designated solvent. The procedure is as follows.
Line Purging - End of Day Procedure
Place all the buffer lines that needs to be purged in an empty bottle/container.
Run the following method to purge the line with air, flush the line with water, and then purge the line with air again. This method can be saved on the system for future use.
Leave the solvent line in the empty container until the beginning of the next shift.
Action | Inlet 1 | Flow | Volume | Notes |
Prime w1 | Buffer # | 10 | 5 | Draw 5 mL of air through the solvent line and push through to top waste. |
Wait | Manual Resume | Program will automatically pause. Transfer the solvent line from an empty container to a bottle of clean water. | ||
Prime w1 | Buffer # | 10 | 10 | Draw 10 mL of water through the solvent line and push through to top waste |
Wait | Manual Resume | Program will automatically pause. Transfer the solvent line back to the empty container. | ||
Prime w1 | Buffer # | 10 | 5 | Draw 5 mL of air through the solvent line and push through to top waste |
Re-Priming - Beginning of the Next Day Procedure
Place the buffer line back into the designated buffer and run the following method. This method can be saved on the system for future use.
The buffer line is now re-priming and ready for the next extraction.
Action | Inlet 1 | Flow | Volume | Notes |
Prime w1 | Buffer # | 10 | 3 | Prime the buffer line and valve 1 with 3 mL of designated buffer. If multiple buffer lines are used, add a prime step for each line used so that all lines are primed |
Prime w1 | Water | 10 | 5 | Clean valve 1 and the top waste line with 5 mL of water |
Air-purge w1 | Air | 10 | 5 | Purge valve 1 and the top waste line with 5 mL of air |