Lumera Site Preparation

This guide helps you prepare you laboratory facility for the installation of the LUMERA 8-Channel cleanup station. Proper site preparation is critical to the successful operation of the system.

This site preparation guide applies for the following system part numbers:

• LUM-92T-UVP

• LUM-96T-UVP

• LUM-92T

• LUM-96T

Customer Support

If you have questions about this guide or preparing your site, contact support@promochrom.com

The following figure shows a typical Lumera automated SPE system.

• The system should be placed on a stable laboratory bench or fume hood with an open space of at least 80 cm wide x 50 cm deep (31.5" x 19.7") to provide access to the front and side of the instrument for fraction collection and sample loading.

• There should be space underneath the bench for the placement of waste containers. The instrument must be elevated well above the waste container to allow for proper drainage.

• Proper ventilation is required if toxic and volatile buffers are to be used.

Refer to the following power requirements when preparing your laboratory

Electrical Safety

Follow all local electrical safety requirements in preparing you laboratory

Power Source

All systems require a dedicated power source. The receptacles from this power source should be accessible to the operator for easy disconnection from the source. If you are unsure what power source comes with your system, please contact support@promochrom.com.

Plug/Receptacle Types

Laboratory Location	Plug Type
Canada, United States, Mexico, Japan	NEMA 5-15P
Other	CEE 7/VII "Schuko" 1.6A

Air Quality

Ensure that the laboratory is not exposed to excessive dust

Humidity

Ensure that the relative humidity of the laboratory is between 20% and 80%, noncondensing

Air flow

Ensure that air flow from heating or air-conditioning diffusers is not directed on the system.

Temperature

The ambient temperature must be from 2 to 40 C (35.6 to 104 F).

The waste management system is a gravity-driven drainage system that effectively collects and removes any waste.

There should be space underneath the bench for the placement of waste container. The instrument must be elevated well above the waste container to allow for proper drainage.

To maintain proper drainage and leak control, ensure that the system is on a stable, level, surface. The waste tubing must continuously be on a downwards angle to avoid flooding the system.

Laboratory Glassware and Accessories

• Buffer bottles with GL45 caps

• 15 mL or 50 mL conical bottom centrifuge tubes for fraction collection

• Protein purification cartridges for method validation

• 500 mL squeeze bottle for cleaning

• Clean sample bottles

• 4L or larger waste container

Solvents & Reagents

• DI Water, HPLC grade or better

• Other reagents and solvents required for method validation