SPE-03 Site Preparation

This guide helps you prepare you laboratory facility for the installation of the SPE-03 automated SPE system. Proper site preparation is critical to the successful operation of the system.

This site preparation guide applies for the following system part numbers:

• SPE03-P-PTFE

• SPE03-P-PE

Related Information

• SPE03 Setup video

• SPE03 Operational guidelines

• SPE03 User Manual

Customer Support

If you have questions about this guide or preparing your site, contact support@promochrom.com

The following figure shows a typical SPE-03 automated SPE system.

• The system should be placed on a stable laboratory bench or fume hood with an open space of at least 80 cm wide x 50 cm deep (31.5" x 19.7") to provide access to the front and side of the instrument for fraction collection and sample loading.

• There should be space underneath the bench for the placement of waste containers. The instrument has to be elevated well above the waste container to allow for proper drainage.

• Proper ventilation if toxic and volatile solvents are to be used.

Refer to the following power requirements when preparing your laboratory

Electrical Safety

Follow all local electrical safety requirements in preparing you laboratory

Power Source

All systems require a dedicated power source. The receptacles from this power source should be accessible to the operatory for easy disconnection from the source. If you are unsure what power source comes with your system, please contact support@promochrom.com

Plug/Receptacle Types

Laboratory Location	Plug Type
Canada, United States, Mexico, Japan	NEMA 5-15P
Other	CEE 7/VII "Schuko" 1.6A

Air Quality

Ensure that the laboratory is not exposed to excessive dust

Humidity

Ensure that the relative humidity of the laboratory is between 20% and 80%, noncondensing

Air flow

Ensure that aire flow from heating or air-conditioning diffusers is not directed on the system.

Temperature

The ambient temperature must be from 4 to 40 C (39.2 to 104 F).

The waste management system is a gravity-driven drainage system that effectively collects and removes any waste.

There should be space underneath the bench for the placement of waste containers. The instrument has to be elevated well above the waste container to allow for proper drainage.

To maintain proper drainage and leak control, ensure that the system is on a stable, level, surface. The waste tubing must continuously be on a downwards angle to avoid flooding the system.

The SPE03 comes with 1.5m of 1/4" PUR tubing with adapters to fit 1/4" or 6mm tubing.

Requires:

• Customers must supply the required compressed gas source needed for the application

• There must be a gas regulator between the gas source and the SPE03 set to ~25 psi for setup

• The gas regulator must have a connection that can fit the 1/4" or 6mm tubing.

Required for Installation:

• Customers must supply the required compressed gas source needed for the application

• There must be a gas regulator between the gas source and the SPE03 set to ~25 psi for setup

• The gas regulator must have a connection that can fit the 1/4" or 6mm tubing.

Laboratory Glassware and Accessories

• Solvent bottles with GL45 cap. Quantity depends on the total number of solvents required by the method.

• 15 mL or 50 mL conical bottom centrifuge tubes for fraction collection

• SPE cartridges or disks for system cleaning and method validation

• 500 mL squeeze bottle for cleaning

• Clean sample bottles.

• 1-2 4L or larger waste containers

Solvents & Reagents

• Methanol, HPLC grade or better

• DI Water, HPLC grade or better

• Other reagents and solvents required for method validation