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SPE-03 Software Controls

SPE-03 Software Controls

The SPE-03 has a simple touch interface that can store up to 100 methods with 100 lines each. Controls are intuitively designed and arranged as shown below:

Flow rate limits (maximum):

  • Rinse = 70 mL/min

  • Other Actions (not rinse) with cartridges = 20 mL/min

  • Other Actions (not rinse) with disks = 70 mL/min

  • Please contact PromoChrom support at support@promochrom.com if you plan to exceed these limits.

Creating a New Method

  1. Tap the “Save As” icon.

  2. Input the new method name and description and tap “OK”.

  3. The new method is now ready to be run.

Delete Step

Select the step you wish to delete and tap the red “X” icon.

Insert Step

  1. Select the step you wish to insert above and tap the green “+” icon.

  2. The method editing page is displayed. Values are populated based on the selected step.

  3. Choose the new action and parameters, then tap “OK”.

  4. The new step is now inserted before the previously selected step. If the “Add Air Purge” is selected, an additional Air-Purge step is added.

Edit Existing Steps

  1. Select the step you want to edit and tap the pencil icon.

  2. The method editing page is displayed. Values are populated based on the selected step.

  3. Edit the step parameters, then tap “OK”.

  4. The step parameters have been updated.

Add a New Step at the End of a Method

  1. Tap “Add New”, which is the bottom line of the method.

  2. The method editing page is displayed. Default values are populated.

  3. Edit the step parameters, then tap “OK”.

  4. The new step is now appended to the end of the method.

Save Method

Tap the “Save” icon to save your changes.

Select Samples

  1. Use the sample checkboxes to choose which samples to run. (minimum one group of 4).

  2. Sample numbering corresponds to the port numbers on each side of the instrument.

Settings

  1. Tap the “Settings” icon to bring up the settings window. Enabled when the system is idle or paused.

Use the “Direct Control” buttons to directly control the pump and tray. This can be used when troubleshooting to handle blocked SPE columns or during a pause to remove collected fractions.

Direct Control or Setting

Result or Action

Pump Draw/Push

Draws or pushes the pump syringes. Can be used to test the pump function.

Tray in/out

Moves the tray in or out. Can be used to test the tray function.

Shaker On/Off

Turns the resonators on and off. Can be used to test shaking resonator function.

Shaker Level

Sets the resonators’ shaking strength. Level 3 is recommended for most applications.

Show run info

Checking will show the information on the bottom of the SPE03 program screen during a run. The information includes how much sample has been added and more.

Shake during rinse

Checking will allow the resonators to turn on during rinsing and rinsate delivery. By default, this box is checked.

Ramp nitrogen

Checking will enable the N2 flow rate to ramp incrementally every 10 seconds for the first 30 seconds of an N2 blow time-based action step.

Troubleshoot

Checking will allow the SPE-03 to bypass initialization when powered on. This box should always remain unchecked except when actively troubleshooting.

Sample Draw Speed

Can be increased or decreased using the arrows.

Declogging Interval

The purpose of this auto-declogging feature is to temporarily clear inline filters and anti-clogging tips of built-up debris coming from samples. The Declogging Interval feature works by drawing up 3mL of sample and then pushing it back into the respective sample bottles at a flow rate of 15mL/min.

  1. Open the “Settings” tab from the SPE-03 main screen.

  2. Change the “Declogging Interval” value from 0

The Declogging Interval will automatically operate whenever Inlet 1 is set to Sample (e.g., Add Sample, Collect from sample).

  • It can be set to run at regular intervals from every 5mLs to every 255mLs.

  • For example, if the declogging interval was set to 200mL and 1L of a sample was being run, it would activate at 200mL, 400mL, 600mL, 800mL, and 1L.

Start

  1. Once you have selected your method and samples, press the “start” button to begin the run.

  2. The “pause” button becomes visible, the current step is highlighted, the time begins countdown, and the run status is displayed at the bottom.

Pause

Pausing allows users to perform actions like changing SPE cartridges, removing blockage, and controlling the pump/tray through the settings window.

  1. After pressing the pause button, the system will wait until the current stroke is complete.

  2. Refer to the status displayed at the bottom of the screen and refrain from doing anything until the system has fully paused.

  3. After the system has paused, pressing the start button will resume the run. Pressing stop will exit the run.

Stop

  1. Stopping will fully exit the run.

  2. After pressing the stop button, the system will wait until the current stroke is complete. Refer to the status displayed at the bottom of the screen and refrain from doing anything until the system has fully stopped.

Start from Anywhere

A method can be started from any step.

This function is useful for situations where a method was stopped partway through, but it is still desirable to complete the remaining steps of the method. The Log feature can remind operators which step was running before the shutdown, and the following steps will allow the user to finish the run from there.

  1. When launching the software again, an “Incomplete method run detected!” warning will pop up.

    1. Tap “Yes” if you would like to return to the last step before the unexpected shutdown or restart and continue to follow the steps below.

    2. Tap “No” to return to normal operations.

  2. The Recovery Advisor window will then open. Record the volume remaining in the step.

  3. Tap “OK” to proceed to the corresponding step.

  4. Then tap the blue “Start” button to proceed to step editing.

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